Data loading

Functions for loading reference data

load_aba_data()

Define the path to voxel data and load lazily if required

load_mousebrain_data()

Load the mousebrain data (LaManno & Siletti et al. 2020)

Marker discovery

Functions to find brain structure markers

structure_markers()

Find differentially expressed features for all structures on a given annotation level

variable_genes()

Finds variable features for all structures on a given annotation level

Reference mapping

Functions to perform comparisons with different references

voxel_map()

Project single cell gene expression data to spatial expression patterns in the mouse brain

brainspan_map()

Project single cell gene expression data to BrainSpan

mousebrain_map()

Project single cell gene expression data to developing mouse brain data (LaManno & Siletti et al. 2020)

reference_map()

Compare a query scRNA-seq dataset with a reference

Deconvolution

Functions to perform reference-based deconvolution of RNA-seq data

deconvolute()

Devonvolute bulk RNA-seq data based on reference data

Visualization

Plotting functions to visualize marker expression and mapping results

plot_annotation()

Plot brain structure annotation

plot_expression()

Plot gene expression across the mouse brain

plot_expression_3d()

Plot gene expression across the mouse brain in 3D

plot_map()

Plot similarity map of single cells

plot_map_3d()

Plot similarity map of single cells to voxels in 3D

plot_structure_similarity()

Plot similarity to brain structures

no_legend()

Remove legend from plot

no_text()

Remove text from plot

no_x_text()

Remove x-axis text from plot

no_y_text()

Remove y-axis text from plot

Utilities

Utility functions for plotting and summarizing results

summarize_groups()

Summarize data over groups

summarize_structures()

Summarize data over structures

assign_cells() assign_to_structure()

Assign cells to structure

as_matrix()

Convert dataframe to matrix

no_legend()

Remove legend from plot

no_text()

Remove text from plot

no_x_text()

Remove x-axis text from plot

no_y_text()

Remove y-axis text from plot